Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences HEPES BioUltra for molecul50G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt dry powder | 103404-87-1 | 10PAK
HEPES hemisodium salt dry powder | Mol Wt: 249.3 | 103404-87-1 | 10PAK
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt >=99% (titration) | 103404-87-1 | 100G
HEPES hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 249.3 | 103404-87-1 | 100G
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt | 103404-87-1 | | 25g
HEPES hemisodium salt | Purity: 99% | MW: 249.3 | 103404-87-1 | 25g
Bioworld EGTA 0.5M, PH 8.0,250ML
EGTA 0.5M, pH 8.0, DNase and RNase Free,250ml.EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline1L
Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures as well as during sample preparation of cell lysates for use in general molecular biology applications.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer 5x1L
TRIS Tris-Borate-EDTA buffer 5x1L
Sigma Aldrich Fine Chemicals Biosciences BIS TRIS propane BioXtra 9100G
BIS TRIS propane BioXtra 9100G
Asuragen Inc TSMIII Storage Buffer, 50 mL, 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III, pH 7.0, -15 to -30°C
Asuragen TSMIII Storage Buffer is a specialized buffered solution designed to maintain stability for protein-coated nucleic acids. This reagent is ideal for the dilution of Asuragen's Armored products, ensuring the integrity of these sensitive biological materials through multiple freeze-thaw cycles. The stability provided by this storage buffer allows for flexible and scalable testing processes in various molecular assay applications.
- Volume: 50 mL
- Composition: 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III
- pH: 7.0
- Primary application: Dilution of Asuragen's Armored products
- Key benefit: Provides stability to protein-coated nucleic acids
- Storage stability: Withstands multiple freeze-thaw cycles
- Recommended storage temperature: -15 to -30 °C (secondary)
- Testing process benefit: Enables flexible and scalable testing processes
Pickering Laboratories Inc CHEM LITHIUM ELUENT 4X1QT/CS
Lithium Eluant, pH 7.50, case of 4 (950 mL/bottle) Amino Acid Analysis.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML