Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 100 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 250g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 250g
Sigma Aldrich Fine Chemicals Biosciences PIPES BioPerformance Certi500G
PIPES BioPerformance Certi500G
LICOR Odyssey MES SDS Running Buffer Powder (pack of 5)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
![Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75060-25.0.JPG-250.jpg)
Research Products International Corp HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS], 25 Grams
- Substance Name: HEPPS [N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)] [EPPS]
- CAS Number: 16052-06-5
- Molecular Formula: C9H20N2O4S
- Molecular Weight: 252.3
Research Products International Corp TES, Sodium Salt, 25 G
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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Biotang Inc MOPS Buffered Saline 5X, pH 7.0. 125 mM MOPS, 750 mM Sodium Chloride, pH 7.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-570.
MOPS Buffered Saline 5X, pH 7.0. 125 mM MOPS, 750 mM Sodium Chloride, pH 7.0. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-570.
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 50 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It has a pKa of 7.84 at 2.0 mM buffer concentration and exhibits a relatively high ability to bind copper(II). It is for research use only.
- Zwitterionic buffer
- Working pH range: 7.1-8.5
- pKa of 7.84 at 2.0 mM buffer concentration
- Relatively high ability to bind copper(II)
Medchemexpress LLC Bicine | 150-25-4 | 98.7% | 25 G
Bicine is a buffer utilized in physiological research. It is a derivative of the amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection.
- Buffer utilized in physiological research
- Derivative of the amino acid glycine
- Enhances the sensitivity of guanosine enzyme histochemical detection
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255312.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
![Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T31020-100.0.JPG-250.jpg)
Research Products International Corp TES, Sodium Salt, [2-Tris(hydroxymethyl) methyl-2-amino-1-ethanesulfonic acid, sodium salt], 100 Grams
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Sigma Aldrich Fine Chemicals Biosciences POPSO hydrate >=99% (titration) | 68189-43-5 (anhydrous) | 100G
POPSO hydrate >=99% (titration) | Purity: >=99% (titration) | 68189-43-5 (anhydrous) | 100G